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Disabled homolog 2 is required for migration and invasion of prostate cancer cells
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《医学前沿(英文)》 2015年 第9卷 第3期 页码 312-321 doi: 10.1007/s11684-015-0401-3
Disabled homolog 2 (DAB2) is frequently deleted or epigenetically silenced in many human cancer cells. Therefore, DAB2 has always been regarded as a tumor suppressor gene. However, the role of DAB2 in tumor progression and metastasis remains unclear. In this study, DAB2 expression was upregulated along with human prostate cancer (PCa) progression. DAB2overexpression or knockdown effects in LNCaP and PC3 cell lines were verified to address the biological functions of DAB2 in PCa progression and metastasis. LNCaP and PC3 cell lines were generated from human PCa cells with low and high metastatic potentials, respectively. The results showed that DAB2 shRNA knockdown can inhibit the migratory and invasive abilities of PC3 cells, as well as the tumorigenicity, whereas DAB2 overexpression enhanced LNCaP cell migration and invasion. Further investigation showed that DAB2 regulated the cell migration associated genes in PC3 cells, and the differential DAB2 expression between LNCaP and PC3 cells was partly regulated by histone 4 acetylation. Therefore, DAB2 may play an important role in PCa progression and metastasis.
Jingyue Zhang, Yawen Song, Qianqian Shi, Li Fu
《医学前沿(英文)》 2021年 第15卷 第5期 页码 649-656 doi: 10.1007/s11684-021-0830-0
null
《医学前沿(英文)》 2017年 第11卷 第3期 页码 410-422 doi: 10.1007/s11684-017-0527-6
Aberrant expression of annexin A2-S100A10 heterotetramer (AIIt) associated with PML/RARα fusion protein causes lethal hyperfibrinolysis in acute promyelocytic leukemia (APL), but the mechanism is unclear. To facilitate the investigation of regulatory association between ANXA2 and promyelocytic leukemia/retinoic acid receptor a (PML/RARα) fusion protein, this work was performed to determine the transcription start site of ANXA2 promoter with rapid amplification of 5′-cDNA ends analysis. Zinc-induced U937/PR9 cells expressed PML/RARα fusion protein, and resultant increases in ANXA2 transcripts and translational expressions of both ANXA2 and S100A10, while S100A10 transcripts remained constitutive. The transactivation of ANXA2 promoter by PML/RARα fusion protein was 3.29±0.13 fold higher than that by control pSG5 vector or wild-type RARα. The overexpression of ANXA2 in U937 transfected with full-length ANXA2 cDNA was associated with increased S100A10 subunit, although S100A10 transcripts remained constitutive. The tPA-dependent initial rate of plasmin generation (IRPG) in zinc-treated U937/PR9 increased by 2.13-fold, and cell invasiveness increased by 27.6%. Antibodies against ANXA2, S100A10, or combination of both all remarkably inhibited the IRPG and invasiveness in U937/PR9 and NB4. Treatment of zinc-induced U937/PR9 or circulating APL blasts with all-trans retinoic acid (ATRA) significantly reduced cell surface ANXA2 and S100A10 and associated reductions in IRPG and invasiveness. Thus, PML/RARα fusion protein transactivated the ANXA2 promoter to upregulate ANXA2 and accumulate S100A10. Increased AIIt promoted IRPG and invasiveness, both of which were partly abolished by antibodies against ANXA2 and S100A10 or by ATRA.
关键词: annexin A2-S100A10 heterotetramer PML/RARα fusion protein plasmin cell invasion acute promyelocytic leukemia
Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and
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《医学前沿(英文)》 2014年 第8卷 第4期 页码 456-463 doi: 10.1007/s11684-014-0353-z
microRNAs (miRNAs) have played a key role in human tumorigenesis, tumor progression, and metastasis. On the one hand, miRNAs are aberrantly expressed in many types of human cancer; on the other hand, miRNAs can function as tumor suppressors or oncogenes that target many cancer-related genes. This study aimed to investigate the effects of miRNA-200c (miR-200c) on the biological behavior and mechanism of proliferation, migration, and invasion in the prostate cancer cell line Du145. In this study, Du145 cells were transfected with miR-200c mimics or negative control miR-NC by using an X-tremeGENE siRNA transfection reagent. The relative expression of miR-200c was measured by RT-PCR. The proliferation, migration, and invasion abilities of Du145 cells were detected by CCK8 assays, migration assays and invasion assays, respectively. The expressions of ZEB1, E-cadherin, and vimentin were observed by western blot. Results showed that DU145 cells exhibited a high expression of miR-200c compared with immortalized normal prostate epithelial cell RWPE-1. Du145 cells were then transfected with miR-200c mimics and displayed lower abilities of proliferation, migration, and invasion than those transfected with the negative control. The protein levels of ZEB1 and vimentin were expressed at a low extent in Du145 cells, which were transfected with miR-200c mimics; by contrast, E-cadherin was highly expressed. Hence, miR-200c could significantly inhibit the proliferation of the prostate cancer cell line Du145; likewise, miR-200c could inhibit migration and invasion by epithelial-mesenchymal transition.
关键词: miR-200c proliferation migration invasion prostate cancer Du145 cell ZEB1
Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,
《医学前沿(英文)》 2009年 第3卷 第3期 页码 303-308 doi: 10.1007/s11684-009-0060-3
《环境科学与工程前沿(英文)》 2023年 第17卷 第11期 doi: 10.1007/s11783-023-1741-x
● Spartina abundance decreases over time by chemical control.
关键词: Plant invasion Biodiversity Meta-analysis Restoration Salt marshes Treatment timing
林鹏
《中国工程科学》 2003年 第5卷 第6期 页码 33-38
简述红树林湿地在滨海湿地中的地位,红树林的种类和分布,红树林类群的界定,湿地保护中的重要性,红树林的生态、社会和经济价值。着重讨论中国红树林生态工程中面临的四个问题:1.红树林生态恢复的关键是宜林地的选择标准;2.红树林区海堤修复工程的生态模式;3.防止引种时带进外来有害物种的生态入侵;4.要以最小的资源环境代价,谋求经济社会最大限度的发展,从而促进红树林湿地的保护与发展。
唐廷贵,张万钧
《中国工程科学》 2003年 第5卷 第3期 页码 15-20
1963年以来,我国先后从国外引进3种大米草,分布在18000 km海岸带上,经过近40年的引种、驯化和栽培,种植面积已达到36000 hm2。实践证明大米草和互花米草适应于中国海岸带自然环境而正常生长发育,具有明显的促淤造陆、保滩护岸,环境保护等生态功能,并有显著的经济效益,是一种改造滩涂的优良植物,也是一种盐渍土裸地植物群落形成的先锋植物,已作为一个外来种被列入《中国植物志》、《中国高等植物图鉴》和各种地方植物志中。文章认为应客观地评估大米草的功与过。大米草和互花米草繁殖较快的原因是由于富营养化的结果,然而对环境、种群和人并未构成侵害行为,在北方海岸带区域尤未发现任何异常现象,不可认定大米草和互花米草是属于有害的生态入侵种。
Effect of lanthanum chloride on growth of breast cancer cells and regulation of transcription
Xue LI MM , Ping HE MM , Jie XIA MM , Shiwei SONG BS , Jinhai LU BM , Yunde LIU MM ,
《医学前沿(英文)》 2009年 第3卷 第3期 页码 336-340 doi: 10.1007/s11684-009-0053-2
Kai Qu, Chang Liu, Aasef M A Mansoor, Bo Wang, Jincai Chen, Liang Yu, Yi Lv
《医学前沿(英文)》 2011年 第5卷 第4期 页码 434-437 doi: 10.1007/s11684-011-0157-3
关键词: liver abscess locally advanced colon cancer multiorganic invasion
基于改进萤火虫算法的雷达网络最优化部署 Regular Papers
Xue-jun ZHANG, Wei JIA, Xiang-min GUAN, Guo-qiang XU, Jun CHEN, Yan-bo ZHU
《信息与电子工程前沿(英文)》 2019年 第20卷 第3期 页码 425-437 doi: 10.1631/FITEE.1800749
标题 作者 时间 类型 操作
on FASN and MGLL in the regulation of abnormal lipid metabolism and the relationship between tumor invasion
Jingyue Zhang, Yawen Song, Qianqian Shi, Li Fu
期刊论文
is upregulated by PML/RARα fusion protein and promotes plasminogen-dependent fibrinolysis and matrix invasion
null
期刊论文
Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and
null
期刊论文
Effects of hypoxia inducible factor-1alpha siRNA on the invasion of human Hela cells and expression of
Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,
期刊论文
A global synthesis of the effectiveness and ecological impacts of management interventions for species
期刊论文
Effect of lanthanum chloride on growth of breast cancer cells and regulation of transcription
Xue LI MM , Ping HE MM , Jie XIA MM , Shiwei SONG BS , Jinhai LU BM , Yunde LIU MM ,
期刊论文
Pyogenic liver abscess as initial presentation in locally advanced right colon cancer invading the liver, gallbladder, and duodenum
Kai Qu, Chang Liu, Aasef M A Mansoor, Bo Wang, Jincai Chen, Liang Yu, Yi Lv
期刊论文